von Ebner타액선 선포세포의 전기생리학적 특성에 관한연구 - patch clamp를 위한 von Ebner 선포세포 분리방법의 개발 -
The study of electrophysiological characteristics of acinar cells of von Ebner salivery gland:A dissociation method for patch clamp
소속 상세정보
김경년/Kyung-Nyun Kim
KMID : 0362720000380040378
Abstract
The microenvironments of taste cells in circumvallate and foliate papillae are greatly influenced
by secretions from the von Ebner salivary glands(vEGs). The vEGs are associated with the cirumvallate
and tne foliate papillae and their secretions may have a strong impact on gustatin. This study
is designed to find a method for dissociationg acinar cells suitable for patch clamping of the
vEGs acinar cells.
The single cells or clusters of vEGs acinar cells were obtained by sequential trypsin and collagenase
treatment. The ionic currents of resting and voltage-activated state were recorded with whole
cell patch clamp mode. After the enzyme dissociation procedure, the number and quality of salivary
acinar cells from the vEGs were good enough for patch clamp experiments. The whole cell currents
were mainly composed of outward currents. Their current-voltage relationship showed a strong outward
rectification
The dissociation procedure were found very satisfactory and it enabled us to study of the vEGs
physiology to better understand the perireceptor mechanism in circumvallate and foliate papillae,
also it enabled us to understand the excitation-secretion mechanisms in serous salivary gland.
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